• USA Home
  • SAB1402151 - Monoclonal Anti-COL1A1 antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
SAB1402151 Sigma-Aldrich

Monoclonal Anti-COL1A1 antibody produced in mouse

clone 3G3, purified immunoglobulin, buffered aqueous solution

Synonym: OI4

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, CO-CP, Primary Antibodies
conjugate   unconjugated
clone   3G3, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  indirect ELISA: suitable
  western blot: 1-5 μg/mL
species reactivity   human, mouse
mol wt   antigen ~35.79 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG3κ
antibody product type   primary antibodies
NCBI accession no.   NM_000088
UniProt accession no.   P02452
Gene Information   human ... COL1A1(1277)

Description

General description

The COL1A1 (collagen type I α 1 chain) gene codes for the pro-α1 chains of type I collagen. The triple helix of the type I collagen is composed of two α chains and this protein is localized to connective tissues and predominantly expressed in bone, cornea, dermis and tendon. COL1A1 is mapped to human chromosome 17q21.33. 90% of the bone matrix comprises of collagen type I.

Immunogen

COL1A1 (NP_000079.1, 1021 a.a. ~ 1108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
EGSPGRDGSPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAGKSGDRGETGPAGPAGPVGPVGARGPAGPQGPRGDKGETGEQGDRGIK

Physical form

Solution in phosphate buffered saline, pH 7.4

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Proteins belonging to the collagen family, supports and nourishes body tissues such as tendons, cartilage, skin, and sclera of the eye. Mutation in COL1A1 (collagen type I α 1 chain) gene is associated with a number of connective tissue disorders such as Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, osteogenesis imperfecta types I-IV, idiopathic osteoporosis and Caffey Disease. The COL1A1 is associated with a particular type of skin tumor called dermatofibrosarcoma protuberans which is due to over expression of growth factors. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?