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  • SAB2700282 - Anti-GPC1 antibody produced in rabbit

SAB2700282 Sigma-Aldrich

Anti-GPC1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: FLJ38078, GPC1, glypican

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, GP-GP, Primary Antibodies
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   ELISA: suitable
  flow cytometry: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: 500-3000
species reactivity   human, mouse
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
Featured Industry   Research Pathology
NCBI accession no.   NP_002072
UniProt accession no.   P35052
Gene Information   human ... GPC1(2817)


General description

Glypican 1 (GPC1) is located at 2q37.3 on the human chromosome. It belongs to the family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycan. GPC1 is localized in the vascular system. GPC1 consists of an N-terminal secretory signal peptide, 14 conserved cysteine residues, a heparan sulfate (HS) attachment domain near the C terminus and a hydrophobic domain for attachment of the glycosylphosphatidylinositol (GPI) at the C terminus.


Recombinant protein encompassing a sequence within the center region of human Glypican 1.


Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, FACS: 1:50-1:200, ICC/IF: 1:100-1:1000, IHC-F: Assay-dependent dilution, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Physical form

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Glypican 1 (GPC1) plays an important role in cell growth, differentiation and morphogenesis. It also plays a role in cell migration, adhesion, anti-coagulation, lipoprotein metabolism and modulation of growth factors. GPC1 exhibits chaperone-like activity by restoring the binding activity of oxidized VEGF165 (vascular endothelial growth factors-165). Overexpression of GPC1 results in over accumulation of β-amyloid protein (Aβ) in the Alzheimer′s disease (AD) brain. Mutation in this gene is associated with Niemann-Pick C1 disease.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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