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  • SAB2700282 - Anti-GPC1 antibody produced in rabbit

SAB2700282 Sigma

Anti-GPC1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: FLJ38078, GPC1, glypican



Related Categories Alphabetical Index, Antibodies, GP-GP, Primary Antibodies
species reactivity   mouse, human
application(s)   ELISA: suitable
  flow cytometry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  indirect immunofluorescence: suitable
  western blot: 500—3000
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... GPC1(2817)
biological source   rabbit
conjugate   unconjugated
Featured Industry   Research Pathology
NCBI accession no.   NP_002072



Recombinant protein encompassing a sequence within the center region of human Glypican 1.

Physical form

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.


Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, FACS: 1:50-1:200, ICC/IF: 1:100-1:1000, IHC-F: Assay-dependent dilution, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

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Biochem/physiol Actions

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq]

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  1
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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