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SAB2702159 Sigma-Aldrich

Monoclonal Anti-RPA3 antibody produced in mouse

Synonym: REPA3

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RO-RP
conjugate   unconjugated
clone   14.2
  monoclonal
biological source   mouse
application(s)   immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human, mouse
mol wt   14 kDa
form   liquid
shipped in   wet ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   1 mg/mL
UniProt accession no.   P35244
Gene Information   human ... RPA3(6119)

Description

Immunogen

Full-length human RPA-14 expressed in E. coli..

Application

For IP: Use at a concentration of 0.1 - 2 μg/mL. For WB: Use at a concentration of 0.1 - 2 μg/mL. Predicted molecular weight: 14 kDa. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.

Physical form

Phosphate-buffered saline, pH 7.4, containing no preservatives

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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