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SAB3300047 Sigma-Aldrich

Monoclonal Anti-TUBB3 antibody produced in mouse

clone 5H2, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Anti-β-4, Anti-MC1R, Anti-TUBB4

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Embryonic Stem Cells, Antibodies for Stem Cell Biology,
conjugate   unconjugated
clone   5H2, monoclonal
biological source   mouse
application(s)   western blot: suitable
species reactivity   human
mol wt   antigen 50.3 kDa
form   buffered aqueous glycerol solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
antibody product type   primary antibodies
NCBI accession no.   NM_006086
UniProt accession no.   Q13509
Gene Information   human ... TUBB3(10381)

Description

Immunogen

Recombinant protein expressed in E. coli corresponding to amino acids 1-150 of human TUBB3

Target description

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For β-tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic β-tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules.

Physical form

Solution in phosphate buffered saline containing 1% bovine serum albumin, 50% glycerol, and 0.02% sodium azide.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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