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SAB3300072 Sigma-Aldrich

Monoclonal Anti-α-Actinin antibody produced in mouse

clone 7A4, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Anti-FLJ40884, Anti-FLJ54432

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Embryonic Stem Cells,
conjugate   unconjugated
clone   7A4, monoclonal
biological source   mouse
application(s)   indirect immunofluorescence: suitable
  western blot: suitable
species reactivity   human
mol wt   antigen 102.9 kDa
form   buffered aqueous glycerol solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
antibody product type   primary antibodies
NCBI accession no.   NM_001102
UniProt accession no.   P12814
Gene Information   human ... ACTN1(87)

Description

Immunogen

Recombinant protein expressed in E. coli corresponding to amino acids 650-893 of human α-actinin

Target description

α-Actinins belong to the spectrin gene superfamily, which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. α-Actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

Physical form

Solution in phosphate buffered saline containing 1% bovine serum albumin, 50% glycerol, and 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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