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  • SAB3500356 - Anti-MDA5 antibody produced in rabbit

SAB3500356 Sigma-Aldrich

Anti-MDA5 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Interferon-induced with helicase C domain protein 1, Anti-IFIH1, Anti-Melanoma differentiation-associated protein 5



Related Categories Alphabetical Index, Antibodies, Antibodies with Corresponding Blocking Peptides, K-M, MB-ME,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunofluorescence: suitable
  immunohistochemistry: suitable
  indirect ELISA: suitable
  western blot: suitable
species reactivity   human, mouse
mol wt   predicted mol wt 113 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
NCBI accession no.   NP_071451
UniProt accession no.   Q9BYX4
Gene Information   human ... IFIH1(64135)


General description

Melanoma differentiation-associated protein 5 (MDA5), also known as interferon induced with helicase C domain 1 (IFIH1), is a cytosolic RNA sensor. It is encoded by the gene mapped to human chromosome 2q24.2. The encoded protein is a member of the retinoic acid-inducible gene I-like receptor family. MDA5 is characterized with both caspase recruitment domain and putative DExH group RNA helicase domains.


MDA5 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human MDA5.


Anti-MDA5 antibody produced in rabbit has been used in western blot analysis.

Physical form

Supplied in PBS with 0.02% sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Melanoma differentiation-associated protein 5 (MDA5) plays a vital role in antiviral innate immune responses. In addition, the encoded protein also activates interferon regulatory factors 3 and 7 and nuclear factor-κB (NF-κB) via a mitochondrial viral signaling protein, inducing antiviral and proapoptotic responses, which is associated with various autoimmune diseases, such as type 1 diabetes (T1D), Graves′ disease (GD), multiple sclerosis (MS), psoriasis and rheumatoid arthritis (RA). Polymorphism in the gene is associated with the development of generalized vitiligo (GV).

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.


The action of this antibody can be blocked using blocking peptide SBP3500356.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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