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  • SAB3701046 - Anti-Mouse IgG IgA IgM (H+L), highly cross adsorbed-Fluorescein antibody produced in goat

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SAB3701046 Sigma-Aldrich

Anti-Mouse IgG IgA IgM (H+L), highly cross adsorbed-Fluorescein antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: FITC

  •  NACRES NA.46

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Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates,
conjugate   fluorescein conjugate
clone   polyclonal
biological source   goat
application(s)   direct immunofluorescence: suitable
  indirect ELISA: suitable
  indirect immunofluorescence: suitable
species reactivity   mouse
form   lyophilized powder
shipped in   wet ice
storage temp.   2-8°C
antibody form   affinity isolated antibody
Quality Level   100
antibody product type   secondary antibodies

Description

General description

Immunoglobulin G (IgG) is a widely expressed serum antibody. It is part of the immunoglobulin family and consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Immunoglobulin M (IgM) exists as a pentamer in the serum and has a high molecular weight. Monomeric IgM has a molecular weight of 190kDa. The pentameric IgM consists of ten μ-heavy chains, ten light chains and a single J-chain which is linked by disulphide bonds. The μ -chain consists of five domains- VH, Cμ1, Cμ2, Cμ3 and Cμ4. Immunoglobulin A (IgA) is found as two subclasses, that is, IgA1 and IgA2. IgA1 has an extended hinge because of the insertion of a duplicated stretch of amino acids, whereas IgA2 lacks this. IgA exists as a monomer or dimer in the secreted form. Dimeric IgA has a molecular weight of 390kDa. This antibody can form multimers and its polymerization is essential for transport across epithelia. IgA is crucial as the first line of defence and has an important role in mucosal immunity.

Specificity

This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by cross adsorptions to remove unwanted specificities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Fluorescein, Anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM. No reaction was obserbed against Bovine, Horse or Human Serum Proteins. This reagent is suitable for the detection of all Mouse isotypes and chain combinations.

Immunogen

Mouse IgG IgA and IgM whole molecule

Physical form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution

Reconstitute with 1.0 mL deionized water (or equivalent).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Physical properties

Antibody format: IgG

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Supplemental Hazard Statements 
EUH032
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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