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  • SAB3701056 - Anti-Mouse IgG IgA IgM (H+L), F(ab′)2 fragment, highly cross adsorbed antibody produced in goat

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SAB3701056 Sigma-Aldrich

Anti-Mouse IgG IgA IgM (H+L), F(ab′)2 fragment, highly cross adsorbed antibody produced in goat

affinity isolated antibody, buffered aqueous solution

  •  NACRES NA.46

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Properties

Related Categories Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates, Primary Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   immunohistochemistry: suitable
  indirect ELISA: suitable
  western blot: suitable
species reactivity   mouse
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   affinity isolated antibody
Quality Level   100
antibody product type   secondary antibodies
concentration   0.98 mg/mL

Description

General description

Immunoglobulins (Igs) or antibodies are Y-shaped molecules which are the secreted form of the B-cell receptor. Igs are composed of two heavy and two light chains. An immunoglobulin possesses variable (V) domains that bind to antigens and constant (C) domains that bind to effector molecules. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. Pepsin cleaves the carboxy-terminal side of the disulfide bonds in the general region of the antibody and this gives rise to the F(ab’)2 fragment. The two antigen-binding arms of the antibody are linked in this fragment. There are five classes of immunoglobulins- IgM, IgA, IgG, IgD and IgE, classified based on their C regions.

Specificity

This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Goat Serum. No reaction was observed against Anti-Goat IgG F(c), Anti-Pepsin or Human Serum Proteins. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations.

Immunogen

Mouse IgG IgA and IgM whole molecule

Physical form

Supplied in 0.1 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Physical properties

Antibody format: IgG F(ab′)2

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

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Peer-Reviewed Papers
15

References

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