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  • SAB4100002 - Monoclonal Anti-GFAP antibody produced in mouse

SAB4100002 Sigma-Aldrich

Monoclonal Anti-GFAP antibody produced in mouse

clone GFAP-B4, hybridoma cell culture supernatant

Synonym: Anti-Glial fibrillary acidic protein



Related Categories Alphabetical Index, Antibodies, GD-GL, Primary Antibodies
conjugate   unconjugated
clone   GFAP-B4, monoclonal
biological source   mouse
application(s)   immunoblotting: 1:100-1:200 using rat PC12 cells
  immunocytochemistry: suitable
  immunofluorescence: 1:20 using human U87 cells
species reactivity   human, rat
mol wt   antigen predicted mol wt 60 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1 mg/mL
NCBI accession no.   NM_002055
UniProt accession no.   P14136
Gene Information   human ... GFAP(2670)
rat ... Gfap(24387)


General description

Glial fibrillary acidic protein (GFAP) is an intermediate-filament protein found in the astrocytes of the central nervous system. The gene encoding it is localized on human chromosome 17q21.


GFAP purified recombinant protein


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Glial fibrillary acidic protein (GFAP) regulates intermediate filament network dynamics, cell morphology and focal adhesions. It maintains myelination and stability of the white matter in the central nervous system.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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