• USA Home
  • SAB4200013 - Anti-Kindlin-3 antibody produced in rabbit

EMAIL THIS PAGE TO A FRIEND
SAB4200013 Sigma-Aldrich

Anti-Kindlin-3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-FERMT3, Anti-Fermitin family homolog 3, Anti-KIND3, Anti-MIG-2, Anti-MIG2B, Anti-UNC-112 related protein 2, Anti-UNC112C, Anti-URP2, Anti-URP2SF

  •  NACRES NA.41

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 5-10 μg/mL using whole extracts of human TF1 cells
species reactivity   human
mol wt   antigen ~72 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q86UX7
Gene Information   human ... FERMT3(83706)

Description

General description

Kindlin-3 is a member of the kindlin family of focal adhesion proteins. The kindlin family consists of three members in vertebrates, kindlin-1, kindlin-2 and kindlin-3 that share high sequence homology and a common structure containing a C-terminal 4.1R, ezrin, radixin, moesin (FERM) domain and a pleckstrin-homology domain. Kindlin-1 and kindlin-2 are widely expressed, kindlin-3 is preferentially expressed in hematopoietic cells, mainly in megakaryocytes and platelets.

Application

Anti-Kindlin-3 antibody produced in rabbit has been used in western blotting and immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Kindlin-3 is required for integrin-mediated leukocyte adhesion to endothelial cells. Mutations in kindlin-3 cause leukocyte adhesion deficiency-III. Kindlin-1, kindlin-2 and kindlin-3 are essential for the regulation of integrin activation by binding the tails of integrin-β subunits. Loss of kindlins results in severe defects in integrin signaling, cell-ECM adhesion and cytoskeletal organization. Deficiency of kindlin-1 results in kindler syndrome, kindlin-2 results in early embryonic lethality due to cardiac developmental problems, and kindlin-3 results in postnatal lethality.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?