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  • SAB4200019 - Anti-TBL1X antibody, Mouse monoclonal

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SAB4200019 Sigma-Aldrich

Anti-TBL1X antibody, Mouse monoclonal

clone TX22, purified from hybridoma cell culture

Synonym: Anti-Transducin-beta-like 1, X-linked, Anti-EBI, Anti-SMAP55, Anti-TBL1

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Oxidative Stress Proteins,
conjugate   unconjugated
clone   TX22, monoclonal
biological source   mouse
application(s)   western blot: 0.2-0.4 μg/mL using HeLa nuclear cell extracts
species reactivity   canine, bovine, human
mol wt   antigen ~55 kDa
form   buffered aqueous glycerol solution
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   purified from hybridoma cell culture
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   O60907
Gene Information   human ... TBL1X(6907)
mouse ... Tbl1x(21372)
rat ... Tbl1x(302711)

Description

General description

Monoclonal Anti-TBL1X (mouse IgG2b isotype) is derived from the hybridoma TX22 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant fusion protein. Transducin β-like protein 1X-linked (TBL1X) belongs to the tryptophan-aspartic acid (WD)-40 repeat proteins. This protein is found as a subunit in the corepressor silencing mediator for retinoid and thyroid receptors (SMRT) complex along with histone deacetylase 3 protein. The TBL1X gene is mapped on the human chromosome at Xp22.31.

Specificity

Monoclonal Anti-TBL1X recognizes human, bovine, and dog TBL1X.

Application

Anti-TBL1X antibody, Mouse monoclonal may be used in immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Transducin β-like protein 1X-linked (TBL1X) gene is universal and is obliterated in late-onset sensorineural deafness which is linked to X-linked syndrome of ocular albinism.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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