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  • SAB4200075 - Anti-VAC14 (C-terminal) antibody produced in rabbit

SAB4200075 Sigma-Aldrich

Anti-VAC14 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-ArPIKfyve, Anti-TAX1-binding protein 2, Anti-TAX1BP2 TAX-reactive protein x, Anti-TAX1BP2, Anti-TRX

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Lipids in Cell Signaling, Primary Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 10-15 μg using A431 cell lysates
  indirect immunofluorescence: 8-16 μg/mL using HeLa cells
  western blot: 1-2 μg/mL using extracts of mouse brain (S1 fraction) or rat brain (S1 fraction)
species reactivity   rat, mouse, human
mol wt   antigen ~82 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1.5 mg/mL
UniProt accession no.   Q08AM6
Gene Information   human ... VAC14(55697)
rat ... Vac14(307842)


General description

VAC14 gene is mapped to human chromosome 16q22.1-q22.2. VAC14 is a scaffold protein and is a component of the PIKfyve protein kinase complex.


Anti-VAC14 (C-terminal) specifically recognizes human, mouse, and rat VAC14.


Anti-VAC14 (C-terminal) antibody produced in rabbit has been used in:
• immunoblotting
• immunoprecipitation
• immunofluorescence

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Mutation in VAC14 gene leads to the development of retinitis pigmentosa (RP).

VAC14 is a regulator of PIP5K3/PIKfyve, a dual specificity kinase.

VAC14 phosphorylates PtdIns(3)P to generate the housekeeping phospholipid PtdIns(3,5)P2. It associates with and upregulate PIKfyve phosphoinositide-5-kinase activity. VAC14-PIP5K3- PtdIns(3,5)P2 pathway is thought to be physiologically linked to insulin activation of glucose transport into the cell. Knockout of the VAC14 gene in mice results in massive neurodegeneration and particularly affected are neurons in the midbrain and of the peripheral sensory system.

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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