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SAB4200080 Sigma-Aldrich

Anti-Vinculin antibody, Mouse monoclonal

clone V284, purified from hybridoma cell culture

Synonym: Anti-CMD1W, Anti-MVCL, Anti-VCL

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   V284, monoclonal
biological source   mouse
application(s)   immunocytochemistry: 4-8 μg/mL using HeLa cells fixed with methanol:acetone
  immunoprecipitation (IP): suitable
  western blot: 0.005-0.01 μg/mL using HeLa total cell extracts
species reactivity   mouse, human, chicken, rat, canine, monkey, rabbit
mol wt   antigen ~117 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
  purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
Quality Level   200
concentration   ~1 mg/mL
UniProt accession no.   P18206
Gene Information   human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

Description

General description

Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hybridoma V284 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with purified human platelet vinculin. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), neuromuscular junctions, myotendinous junctions (skeletal muscle) and the membrane-associated dense bodies of smooth muscle.

Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein.

Immunogen

purified human platelet vinculin.

Application

Anti-Vinculin antibody, Mouse monoclonal has been used:
• to probe blots in immunoblotting
• immunochemical staining
• immunoprecipitation
• immunocytochemistry

Monoclonal Anti-Vinculin antibody produced in mouse has been used in western blotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading. In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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