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  • SAB4200102 - Anti-AS160 (C-terminal) antibody produced in rabbit

SAB4200102 Sigma-Aldrich

Anti-AS160 (C-terminal) antibody produced in rabbit

Antibody Enhanced Validation

~1.5 mg/mL

Synonym: Anti-Akt substrate of 160 kDa, Anti-TBC1D4 (TBC1 domain family, member 4)

  •  NACRES NA.41



Related Categories AS-ATG, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins and Cyclic Nucleotides,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 0.8-1.6 μg/mL using extracts of HEK-293T cells overexpressing human AS160
species reactivity   human
mol wt   antigen ~160 kDa
enhanced validation   recombinant expression
Learn more about Antibody Enhanced Validation
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1.5 mg/mL
UniProt accession no.   O60343
Gene Information   human ... TBC1D4(9882)


General description

AS160 is a protein kinase B (Akt) substrate of 160 kDa, also known as TBC1D4. This gene is mapped to human chromosome 13q22.2 and encodes a Rab guanosine triphosphatase (GTPase)-activating protein (RabGAP). It has a number of insulin-regulatable protein kinase B (AKT) phosphomotifs. AS160 is expressed in many differentiated tissues such as adipocytes and skeletal muscles.


Anti-AS160 (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

AS160 (protein kinase B (Akt) substrate) is known to play an inhibitory role in the transport of glucose transporter 4 (GLUT4) vesicles. AS160 is associated with GLUT4 vesicles in basal states and dissociates in response to insulin. Reduced expression of AS160 in adipocytes has been shown to increase plasma membrane levels of GLUT4 in an insulin-dependent manner.

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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