• USA Home
  • SAB4200103 - Anti-AS160 (C-terminal region) antibody produced in rabbit

EMAIL THIS PAGE TO A FRIEND
SAB4200103 Sigma-Aldrich

Anti-AS160 (C-terminal region) antibody produced in rabbit

Antibody Enhanced Validation

~1.5 mg/mL, affinity isolated antibody

Synonym: Anti-Akt substrate of 160 kDa, Anti-TBC1D4 (TBC1 domain family, member 4)

Purchase

Properties

Related Categories AS-ATG, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins and Cyclic Nucleotides,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 8-16 μg/mL using HEK-293T cells
  western blot: 0.8-1.6 μg/mL using extracts of HEK-293T cells overexpressing human AS160
species reactivity   human
mol wt   antigen ~160 kDa
enhanced validation   recombinant expression
Learn more about Antibody Enhanced Validation
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1.5 mg/mL
UniProt accession no.   O60343
Gene Information   human ... TBC1D4(9882)

Description

General description

TBC1 domain family member 4 (TBC1D4) also known AS160, is a protein kinase B (Akt) substrate of 160 kDa. It is a Rab guanine triphosphatase (GTPase)-activating protein (GAP) protein. It consists of two phosphotyrosine-binding (PTB) domains at the N-terminal and a C-terminal catalytic Rab-GAP domain. This multi-domain protein also possesses Akt phosphorylation sites. The AS160 gene is mapped to human chromosome 13q22.2.

Specificity

Anti-AS160 (C-terminal region) specifically recognizes human AS160.

Application

Anti-AS160 (C-terminal region) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

AS160 protein mediates the translocation of glucose transporter 4 (GLUT4) storage vesicles to the plasma membrane (PM). It is a candidate signaling intermediate, identified as a functional link between the insulin signaling cascade and GLUT4 vesicles. AS160 is known to be phosphorylated by protein kinase B (Akt) on at least six separate sites in response to insulin and is thought to play an inhibitory role in the transport of GLUT4 vesicles. It is associated with GLUT4 vesicles in basal states and dissociates in response to insulin. The inhibitory role of AS160 in the basal state and reduced expression of AS160 in adipocytes has been shown to increase plasma membrane levels of GLUT4 in an insulin-dependent manner. Rab10 has been reported to be a target of AS160, required for insulin-stimulated translocation of GLUT4.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?