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  • SAB4200125 - Monoclonal Anti-Notch3 antibody produced in rat

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SAB4200125 Sigma-Aldrich

Monoclonal Anti-Notch3 antibody produced in rat

~1.0 mg/mL, purified immunoglobulin

Synonym: Anti-CADASIL, Anti-CASIL

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Neurobiology, Antibodies to Neural Proteins, NK-NR,
conjugate   unconjugated
clone   monoclonal
biological source   rat
application(s)   western blot: 2-4 μg/mL using using Jurkat cell extracts
species reactivity   rat, human
mol wt   antigen ~270 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   purified immunoglobulin
isotype   IgG2a
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q9UM47
Gene Information   human ... NOTCH3(4854)
rat ... Notch3(56761)

Description

General description

Monoclonal Anti-Notch3 (rat IgG2a isotype) is derived from the hybridoma NIZ 8G5 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from the immunized rat.
Members of the Notch gene family encode transmembrane receptors. Mammals have four Notch receptors, Notch1 through Notch4. Notch1 and Notch2 have the highest homology with each other, whereas Notch3 and Notch4 are structurally divergent, lacking the transactivation domain that is found in Notch1 and Notch2. The Notch3 receptor possesses 34 extracellular epidermal growth factor (EGF)-like repeats and three cysteine-rich Notch/LIN12 repeats (LIN). It also contains two protein-protein interaction domains, the RAM domain (R), two nuclear localization signals (NLS), six ankyrin (ANK) repeats and a PEST sequence (P) at its cytoplasmic portion. The NOTCH3 gene is mapped to human chromosome 19p13.12.

Specificity

Monoclonal Anti-Notch3 recognizes human and rat Notch3.

Immunogen

human recombinant full length Notch3.

Application

Monoclonal Anti-Notch3 antibody produced in rat is suitable for immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at -20 °C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20 °Cin working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discardedif not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The Notch receptors are critical for various cell fate decisions. The Notch signaling plays a key role in the normal development of many tissues and cell types. It has diverse effects on differentiation, survival and/or proliferation that are highly dependent on the signal strength and cellular context. Variations in the NOTCH3 gene leads to vascular degenerative disease known as cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). In addition, upregulation of the NOTCH3 gene indicates active and relapsing human T-cell acute lymphoblastic leukemia (T-ALL).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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