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  • SAB4200159 - Anti-Glutaredoxin-1 antibody produced in rabbit

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SAB4200159 Sigma-Aldrich

Anti-Glutaredoxin-1 antibody produced in rabbit

buffered aqueous solution

Synonym: Anti-GLRX, Anti-GRX1, Anti-GRX, Anti-TTase-1, thioltransferase-1

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Nitric Oxide Research,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:500-1:1000 using whole extracts of human HepG2 cells
species reactivity   human
mol wt   ~12 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   Ig fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P35754
Gene Information   human ... GLRX(2745)

Description

General description

Anti-Glutaredoxin-1 is produced in rabbit using as immunogen a human glutaredoxin-1 recombinant protein (G5298), conjugated to KLH. GRX1 is found in the cytosol and supplies ribonucleotide reductase with electrons.

Glutaredoxin-1 (Grx1) is encoded by the gene mapped to human chromosome 5q15. The encoded protein is a sulfhydryl disulfide oxidoreductase enzyme with a molecular mass of 12kDa. Grx1 belongs to the oxidoreductase family. Grx is expressed in various types of mammalian cells including human placenta and erythrocytes.

Immunogen

human glutaredoxin-1 recombinant protein conjugated to KLH

Application

Anti-Glutaredoxin-1 antibody produced in rabbit has been used in immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

GRX1 is involved in general disulfide-dithiol exchanges, dehydroascorbate reduction and regulation of transcription factors.

Glutaredoxin (Grx) catalyzes glutathione (GSH)-dependent disulfide reductions when coupled to GSH, NADPH and glucocorticoid receptor (GR). Grx1 is implicated in various cellular processes, such as anti-oxidation, anti-apoptosis and regulation of cell differentiation. Additionally, the encoded protein also protects the retinal pigment epithelial (RPE) cells from H2O2-induced apoptosis by inhibiting protein kinase B (AKT) glutathionylation. Thus, Grx1 can be used as a potential therapeutic target for retinal degenerative diseases.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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