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  • SAB4200193 - Monoclonal Anti-Glyoxalase I antibody produced in rat

SAB4200193 Sigma-Aldrich

Monoclonal Anti-Glyoxalase I antibody produced in rat

~1.0 mg/mL, clone Clone 6F10

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Oxidative Stress Proteins,
conjugate   unconjugated
clone   Clone 6F10, monoclonal
biological source   rat
application(s)   immunocytochemistry: suitable
  western blot: 0.12-0.25 μg/mL using A549, HeLa, SH-SY5Y, COS7 or MDCK cell extracts
species reactivity   human, canine, mouse, rat, monkey
mol wt   ~21 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   purified immunoglobulin
isotype   IgG2b
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q04760
Gene Information   human ... GLO1(2739)
mouse ... Glo1(109801)
rat ... Glo1(294320)


General description

Monoclonal Anti-Glyoxalase I (rat IgG2b isotype) is derived from the hybridoma 6F10 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a mouse Glyoxalase I fusion protein. The glyoxalase system, which consists of glyoxalase I (GLO1), glyoxalase II, and a catalytic amount of reduced glutathione (GSH), is important part of cellular metabolism. GLO1 appears to be ubiquitously expressed in all mammalian cells, suggesting its biological importance.


mouse Glyoxalase I fusion protein


Monoclonal Anti-Glyoxalase I antibody produced in rat has been used in immunoblotting. and immunocytochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The glyoxalase system plays a major role to detoxify α-ketoaldehydes, especially methylglyoxal (MG), that are endogenously formed as a by-product of the triosephosphate isomerase reaction during glycolysis. A member of this system, GLO1 catalyzes the isomerization of a hemithioacetal, comprised of a nonenzymatic adduct of MG and glutathione (GSH), to the corresponding α-d-hydroxyacid thioester and S-D-lactoylglutathione. Studies have suggested that GLO1 may be important for brain function since, GLO1 in the brain is involved in Alzheimer′s disease, autism, anxiety and the regulation of theta oscillations during sleep.

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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