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SAB4200270 Sigma-Aldrich

Anti-PSMB9 antibody, Mouse monoclonal

clone LMP2-37, purified from hybridoma cell culture

Synonym: Monoclonal Anti-LMP2, Monoclonal Anti-PSMB6i, Monoclonal Anti-RING12, Monoclonal Anti-beta1i, Monoclonal Anti-proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)

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Properties

Related Categories Alphabetical Index, Antibodies, PS-PS, Primary Antibodies
conjugate   unconjugated
clone   LMP2-37, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  western blot: 1.0-2.0 μg/mL using extracts of rat spleen
species reactivity   human, mouse, rat
mol wt   antigen 17 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
isotype   IgG2a
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P28065
Gene Information   human ... PSMB9(5698)

Description

General description

Monoclonal Anti-PSMB9 (mouse IgG2a isotype) is derived from the hybridoma LMP2-37 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human PSMB9 recombinant protein. Proteasome 20S subunit beta 9 (PSMB9) is present in the major histocompatibility complex (MHC) class II region. γ-interferon triggers the synthesis of three proteasomal subunits-PSMB9 (β1i), PSMB10 (β2i) and PSMB8 (β5i), which is a part of immunoproteasome.

Immunogen

human PSMB9, recombinant protein.

Application

Monoclonal Anti-PSMB9 antibody produced in mouse has been used in:
• flow cytometry
• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Proteasome 20S subunit beta 9 (PSMB9) is used during antigen presentation. Aberrant levels of PSMB9 is involved in autoimmune disorders such as primary Sjogren′s syndrome and uterine leiomyosarcomas. The proteasome is a multicatalytic proteinase complex, which is involved in the regulation of essential cellular processes such as transcription, cell cycle progression, differentiation, apoptosis and in the control of stress and immune responses.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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