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  • SAB4200274 - Anti-EIF2C2/Ago2 antibody produced in rabbit

SAB4200274 Sigma-Aldrich

Anti-EIF2C2/Ago2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym: Anti-Eukaryotic translation initiation factor 2C, 2, Anti-I2C2, Argonaute-2, Anti-PAZ Piwi domain protein, Anti-Slicer protein



Related Categories Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for RNA Regulation and Translation, Antibodies to RNA Binding Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human EIF2C2/Ago2
species reactivity   human
mol wt   antigen ~97 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
Gene Information   human ... EIF2C2(27161)
mouse ... Eif2c2(239528)
rat ... Eif2c2(59117)


General description

Anti-eukaryotic translation initiation factor 2C 2 (EIF2C2)/protein argonaute-2 (Ago3) is produced in rabbit using as immunogen a synthetic peptide corresponding to an internal region sequence of human EIF2C2/Ago2 conjugated to KLH. Argonaute proteins have a molecular weight of about 100 kDa and are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. Ago subfamily consists of hsAgo1-4 (also known as EIF2C1-4). Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies.


synthetic peptide corresponding to an internal region sequence of human EIF2C2/Ago2, conjugated to KLH. The corresponding sequence is identical in rat and mouse.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Ago2 has endonuclease activity, that cleaves the target RNA in RNAi. Ago2 is regulated at both the transcriptional and posttranslational levels in human breast cancer cell lines. It is implicated in micro-RNA activity and in the tumorigenic progression of breast cancer cell lines.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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