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  • SAB4200276 - Anti-RCC1 (C-terminal) antibody produced in rabbit

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SAB4200276 Sigma-Aldrich

Anti-RCC1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym: Anti-CHC-1, Anti-CHC1, Anti-Chromosome Condensation 1, Anti-Regulator of Chromatin Condensation 1

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies to Epigenetic Regulators, Primary Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 2.5-5.0 μg using lysates of HeLa or HEK-293T cells.
  indirect immunofluorescence: 1-2 μg/mL using paraformaldehyde fixed HeLa cells.
  western blot: 2-4 μg/mL using lysates of HeLa cells
species reactivity   human
mol wt   antigen ~45 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P18754
Gene Information   human ... RCC1(1104)
mouse ... Rcc1(100088)
rat ... Rcc1(682908)

Description

Immunogen

synthetic peptide corresponding to a sequence at the C- terminal region of human RCC1, conjugated to KLH. The corresponding sequence is identical in rat and mouse

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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