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  • SAB4200301 - Monoclonal Anti-Leupaxin antibody produced in mouse

SAB4200301 Sigma-Aldrich

Monoclonal Anti-Leupaxin antibody produced in mouse

clone Leu133, tissue culture supernatant

Synonym: Anti-LDPL, Anti-LPXN



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   Leu133, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  western blot: 1:8000-1:16,000 using human Ramos cell extracts
species reactivity   human, mouse, monkey
mol wt   antigen ~43 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   tissue culture supernatant
isotype   IgM
antibody product type   primary antibodies
UniProt accession no.   O60711
Gene Information   human ... LPXN(9404)
mouse ... Lpxn(107321)


General description

Monoclonal Anti-Leupaxin (mouse IgM isotype) is derived from the hybridoma Leu133 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to a sequence at the N-terminus human Leupaxin conjugated to KLH. Leupaxin is a LIM (Lin11, Isl-1 and Mec-3) domain phosphotyrosine protein preferentially expressed in lymphoid tissues and hematopoietic cells. Leupaxin shares homology with the focal adhesion protein paxillin, in the LIM and LD (leucine/aspartate repeat) protein interaction domains. Leupaxin is expressed in cell types that lack FAK, such as human macrophages.


peptide corresponding to a sequence at the N-terminus human Leupaxin, conjugated to KLH. The corresponding sequence is identical in human leupaxin 1 and 2 isoforms.


Monoclonal Anti-Leupaxin antibody produced in mouse has been used in immunoblotting and immunocytochemistry.

Physical form

Solution containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Leupaxin LIM domains have been shown to mediate localization to focal contacts and LD motifs have been implicated in focal adhesion kinase (FAK) and vinculin binding, resulting in the localization of FAK to focal adhesions. Leupaxin and protein tyrosine kinase 2 β (PYK2) are both preferentially expressed in leukocytes where they regulate cell type-specific signaling complex. Leupaxin can form a complex with PYK2 and PTP-PEST in several cell types including monocytes and osteoclasts and in prostate cancer cells. In osteoclasts, leupaxin has been suggested to be a critical nucleating component of the osteoclast podosomal signaling complex in the adhesion zone. Leupaxin is expressed in prostate cancer and is thought to play a role in adhesion and invasion of prostate carcinoma cells.

Safety & Documentation

Safety Information

NONH for all modes of transport
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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