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  • SAB4200318 - Anti-PRKACA antibody produced in rabbit

SAB4200318 Sigma-Aldrich

Anti-PRKACA antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym: Anti-PKA Cα, Anti-PKACA, Anti-cAMP-dependent protein kinase catalytic subunit α

  •  NACRES NA.44



Related Categories Alphabetical Index, Antibodies, PR-PR, Primary Antibodies
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 0.25-0.5 μg/mL using lysates of HEK-293T cells over-expressing human PRKACA.
species reactivity   human
mol wt   antigen ~40 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P17612
Gene Information   human ... PRKACA(5566)


General description

PRKACA is a serine threonine kinase that phosphorylates various proteins to facilitate several cellular functions. For instance, PRAKACA phosphorylates TRPC1 channel to modulate Ca2+ entry in endothelial tissues and regulates DNA binding functions by phosphorylation of NF-κB p50. PRAKACA also mediates tight junction barrier activity by phosphorylation of claudin-3. Additionally, PRAKACA has been associated with body mass index of asthma patients and the pathogenesis of diabetic osteoporosis
Anti-PRKACA antibody is specific for human PRKACA. In immunoblotting, staining of the PRKACA band is specifically inhibited by the immunizing peptide.


synthetic peptide corresponding to the N-terminal region of human PRKACA, conjugated to KLH. The corresponding sequence differs by 2 and 3 amino acids in mouse and rat, respectively


Anti-PRKACA antibody is suitable for use in western blot (0.25-0.5 μg/mL) using lysates of HEK-293T cells over-expressing human PRKACA.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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