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  • SAB4200340 - Anti-Golph3 (C-terminal) antibody produced in rabbit

SAB4200340 Sigma-Aldrich

Anti-Golph3 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym: Anti-Golgi-associated protein, Anti-GOPP1, Anti-GPP34, Anti-Golgi peripheral membrane protein 1, 34 kDa, Anti-Golgi phosphoprotein 3 (coat-protein), Anti-MIDAS, Anti-Mitochondrial DNA absence factor



Related Categories Alphabetical Index, Antibodies, GM-GO, Primary Antibodies
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells.
  western blot: 0.1-0.2 μg/mL using whole extracts of HEK-293 cells over-expressing human Golph3.
species reactivity   human
mol wt   antigen ~34 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q9H4A6
Gene Information   human ... GOLPH3(64083)
mouse ... Golph3(66629)
rat ... Golph3()


General description

Golgi phosphoprotein 3 (Golph3) is a peripheral membrane protein of the Golgi stack that localizes to the trans-Golgi network. It is conserved from yeast to human. The GOLPH3 gene is mapped to the human chromosome 5p13.3 location.


Anti-Golph3 (C-terminal) recognizes human Golph3.


peptide corresponding to the C-terminal region of human Golph3, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and canine.


Anti-Golph3 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.


Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Golgi phosphoprotein 3 (Golph3) is a phosphatidylinositol-4-phosphate (PtdIns(4)P) binding protein required for Golgi function. It plays a vital role in Golgi trafficking and morphology by interacting with the unconventional myosin 18A (MYO18A) linking Golgi membranes to the F-actin cytoskeleton. GOLPH3 is an oncogene that is commonly targeted for amplification in human cancer and cancer cell lines. Furthermore, Golph3 overexpression is correlated with the hyperactivation of the mammalian target of rapamycin (mTOR) signaling in human cells.

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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