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  • SAB4200341 - Anti-Golph3 (N-terminal) antibody produced in rabbit

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SAB4200341 Sigma-Aldrich

Anti-Golph3 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym: Anti-Golgi-associated protein; Mitochondrial DNA absence factor, Anti-GOPP1, Anti-GPP34, Anti-Golgi peripheral membrane protein 1, 34 kDa, Anti-Golgi phosphoprotein 3 (coat-protein), Anti-MIDAS

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Other Cellular and Organelle Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells
  indirect immunofluorescence: 2.5-5.0 μg/mL using mouse 3T3 cells
  western blot: 1-2 μg/mL using whole extracts of human HeLa cells
species reactivity   mouse, human
mol wt   antigen ~34 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q9H4A6
Gene Information   human ... GOLPH3(64083)
mouse ... Golph3(66629)
rat ... Golph3(78961)

Description

General description

Golph3 (Golgi phosphoprotein 3), also known as coat protein GPP34, is a peripheral membrane protein of the Golgi stack, that localizes to the trans-Golgi network. It is an oncogene that is commonly targeted for amplification in human cancer and in cancer cell lines.

Immunogen

peptide corresponding to the N-terminal region of human Golph3, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and bovine.

Application

Anti-Golph3 (N-terminal) antibody produced in rabbit has been used in:
• immunoprecipitation
• immunofluorescence
• immunoblotting

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Golph3 (Golgi phosphoprotein 3) is important for Golgi trafficking and morphology by interacting with the unconventional myosin MYO18A, linking Golgi membranes to the actin cytoskeleton. It is a phosphatidylinositol-4-phosphate (PtdIns(4)P binding protein required for Golgi function. GOLPH3 overexpression is correlated with hyperactivation of mTOR signaling, in human cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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