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SAB4200361 Sigma-Aldrich

Anti-LC3B antibody, Mouse monoclonal

clone LC3B-6, purified from hybridoma cell culture

Synonym: Monoclonal Anti-microtubule-associated protein 1 light chain 3 beta, Monoclonal Anti-ATG8F, Monoclonal Anti-LC3, Monoclonal Anti-MAP1A/1BLC3, Monoclonal Anti-MAP1LC3B

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Properties

Related Categories Alphabetical Index, Antibodies, L-LL, Primary Antibodies
conjugate   unconjugated
clone   LC3B-6, monoclonal
biological source   mouse
application(s)   immunoprecipitation (IP): 10-20 μg using lysates of human U-87 cells.
  western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human LC3B.
species reactivity   human
mol wt   antigen ~18/16 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2b
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q9GZQ8
Gene Information   human ... MAP1LC3B(81631)

Description

General description

The gene MAP1LC3B (microtubule associated protein 1 light chain 3 β) is a homolog of ATG8 gene, an autophagy-related gene in yeast. The encoded protein is called pro-MAP1LC3B, a pro-form that is cleaved by ATG4 to produce a cytosolic form called MAP1LC3B-I. Anti-LC3B antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma LC3B-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the N-terminal region of human LC3B.

Immunogen

synthetic peptide corresponding to the N-terminal region of human LC3B, conjugated to KLH. The corresponding sequence differs by one amino-acid in rat and mouse.

Application

Anti-LC3B antibody produced in rabbit has been used in:
• western blotting
• immunofluorescence
• confocal laser microscopy
• immunoprecipitation

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

MAP1LC3B-I (microtubule associated protein 1 light chain 3 β-I) conjugates with MAP1LC3B-II before being inserted into both inner and outer membranes of the growing autophagic vesicle during autophagy. The cellular distribution and processing of MAP1LC3B facilitates monitoring of autophagy. Cells that lack MAP1LC3B are found to be defective in the formation of autophagic vesicles, with altered phenotypes associated with the role of autophagy in energy homeostasis and removal of protein aggregates. It can serve as an autophagic marker.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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