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  • SAB4200369 - Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse

SAB4200369 Sigma-Aldrich

Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse

~1.0 mg/mL, clone R2.5254.2, purified immunoglobulin

Synonym: Anti-CD112, Anti-HVEB, Anti-PRR2, Anti-PVRL2, Anti-PVRR2, Anti-Poliovirus receptor-related 2 (herpesvirus entry mediator B)

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   R2.5254.2, monoclonal
biological source   mouse
application(s)   flow cytometry: 5-10 μg/test using using 1x106 MDA-MB-231 cells
  immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells
  indirect ELISA: suitable
  western blot: suitable
species reactivity   rat, human, monkey, mouse
mol wt   antigen ~72 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
Gene Information   human ... PVRL2(19294), PVRL2(5819), PVRL2(308417)


General description

Monoclonal Anti-Nectin-2/PVRL2 (mouse IgG1 isotype) is derived from the hybridoma R2.525.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with 3T3 cells expressing human nectin-2. Nectin-2 is found to be widely expressed in human tissues, including hematopoietic cells.

Nectin-2, also known as poliovirus receptor related protein 2 gene (PRR2), is encoded by the gene mapped to human chromosome 19q13.2. The encoded protein belongs to the nectin protein family and is characterized with an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain.


The antibody has been reported to block cell adhesion and herpes virus entry.


3T3 cells expressing human Nectin-2.


Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse has been used in:
• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry
• flow cytometry

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Nectins act as key elements of adherens junctions and facilitate their formation. Nectin-2 possesses anti-viral property against herpes simplex virus (HSV)-2 infection. In mice, loss of nectin-2 affects sperm production and male-specific infertility. Aberrations in the gene has been associated with the pathogenesis of peripheral T-cell lymphoma (PTCL). Serum levels of nectin-2 can be used as a diagnostic and prognostic marker for colorectal cancer (CRC).

Safety & Documentation

Safety Information

NONH for all modes of transport
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blot Protocol | Immunoblotting Protocol

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Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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