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SAB4200370 Sigma-Aldrich

Monoclonal Anti-PVRL4 antibody produced in mouse

~1.0 mg/mL, clone N4.101.10, purified immunoglobulin

Synonym: Anti-EDSS1, Anti-LNIR, Anti-Nectin-4, Anti-PRR4, Anti-PVRL4, Anti-Poliovirus receptor-related 4, Monoclonal Anti-Nectin-4/PVRL4 antibody produced in mouse

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   N4.101.10, monoclonal
biological source   mouse
application(s)   flow cytometry: 5-10 μg/test using using 1x106 MDA-MB-231 cells
  immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells
species reactivity   human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1.0 mg/mL

Description

General description

Monoclonal Anti-Nectin-4/PVRL4 (mouse IgG1 isotype) is derived from the hybridoma R2.525.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human nectin4-Fc recombinant protein. Nectin-4 belongs to the family of Nectins. It forms homo-cis-dimers and then homo- or hetero-trans-dimers through the extracellular region in a Ca2+-independent manner. Nectin-4 is expressed in placenta, ductal breast carcinoma and lung adenocarcinomas. It is one of the plasma membrane components of adherens junctions.

Immunogen

human Nectin4/Fc recombinant protein

Application

Monoclonal Anti-PVRL4 antibody produced in mouse has been used in immunocytochemistry and flow cytometry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Nectin-4 is involved in cell adhesion through trans homophilic and heterophilic interactions. Mutations in this gene are associated with ectodermal dysplasia-syndactyly syndrome type 1.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Peer-Reviewed Papers
15

References

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