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  • SAB4200394 - Anti-Nestin (C-terminal) antibody produced in rabbit

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SAB4200394 Sigma-Aldrich

Anti-Nestin (C-terminal) antibody produced in rabbit

Antibody Enhanced Validation

~1.5 mg/mL, affinity isolated antibody

Synonym: Anti-NES

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, NE-NI, Primary Antibodies
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 1-2 μg/mL using mouse P19 cells
  western blot: 2-4 μg/mL using lysates of HEK293T cells over-expressing rat nestin.
species reactivity   mouse, rat
mol wt   antigen ~300 kDa
enhanced validation   recombinant expression
Learn more about Antibody Enhanced Validation
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.5 mg/mL
UniProt accession no.   P21263
Gene Information   mouse ... Nes(18008)
rat ... Nes(25491)

Description

General description

Nestin/NES belongs to the intermediate filament (IF) protein family. Its expression is found in immature neuroepithelial and progenitor cell types. This 240 kDa protein contains the shortest head domain (N-terminus) and a long tail domain (C-terminus). Nestin is mapped to human chromosome 1q23.1.

Immunogen

synthetic peptide corresponding to a sequence at the C- terminus of mouse nestin, conjugated to KLH. The sequence is identical in rat nestin.

Application

Anti-Nestin (C-terminal) antibody has been used in immunostaining.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Nestin/NES plays a major role in the modulation of assembly and disassembly of intermediate filaments, which in turn help in the remodeling of the cell along with other structural proteins.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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