• USA Home
  • SAB4200415 - Monoclonal Anti-NEZHA antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
SAB4200415 Sigma-Aldrich

Monoclonal Anti-NEZHA antibody produced in mouse

~1.0 mg/mL, clone NEZHA-1, purified immunoglobulin

Synonym: Anti-CAMSAP3, Anti-Calmodulin regulated spectrin-associated protein family member 3, Anti-KIAA

  •  NACRES NA.41

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   NEZHA-1, monoclonal
biological source   mouse
application(s)   immunoprecipitation (IP): suitable
  indirect immunofluorescence: suitable
  western blot: 2.5-5.0 μg/mL using whole extracts of human SW480 cells
species reactivity   human, canine
mol wt   antigen ~150 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q9P1Y5
Gene Information   human ... CAMSAP3(57662)
mouse ... Camsap3(69697)
rat ... camsap3(556256)

Description

General description

Monoclonal Anti-NEZHA (mouse IgG1 isotype) is derived from the hybridoma NEZHA-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. Nezha (a deity in Chinese mythology) also known as KIAA1543 and calmodulin-regulated spectrin associated protein, is a 1276 amino acid protein that contains one calponin homology (CH) and two coiled coil (CC1 and 2) domains, as well as one DUF1781 (DUF) domain of unknown function. NEZHA is mapped to human chromosome 19p13.

NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) contains a conserved domain called cholecystokinin (CKK) domain at carboxy-terminal, as well as several coiled-coil regions and an calponin homology domain at amino-terminal.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and dog NEZHA.

Application

Monoclonal Anti-NEZHA antibody produced in mouse has been used in immunofluorescence staining.

Monoclonal Anti-NEZHA antibody produced in mouse has been used in:
• immunofluorescence
• immunoprecipitation
• immunofluorescence
• mass-spectrometry-based analysis of a streptavidin pull-down assay
• immunostaining

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

CAMSAP3 (calmodulin-regulated spectrin-associated protein 3)/ NEZHA modulates the minus-end dynamics of microtubules. It helps to maintain neuronal polarity. Absence of this gene results in the formation of supernumerary axon.

NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) facilitates translocation of Golgi vesicles in epithelial cells. In addition, it also helps in positioning the apical-to-basal polarity of microtubules in epithelial cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?