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  • SAB4200434 - Anti-VHL antibody produced in rabbit

SAB4200434 Sigma-Aldrich

Anti-VHL antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym: Anti-HRCA1, Anti-RCA1, Anti-VHL1, Anti-Von Hippel-Lindau tumor supressor

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies for Nitric Oxide Research,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells overexpressing human VHL
  indirect immunofluorescence: 5-10 μg/mL using HeLa cells
  western blot: 1.5-3.0 μg/mL using lysates of HEK-293T cells overexpressing human VHL
species reactivity   human
mol wt   antigen ~30 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P40337
Gene Information   human ... VHL(7428)


General description

Von Hippel-Lindau tumor suppressor (VHL) is encoded by the gene mapped to human chromosome 3p25. The encoded protein is mainly expressed in adult brain and kidney. It is specifically localized on the cell membrane.


synthetic peptide corresponding to a sequence near the C-terminus of human VHL, conjugated to KLH. The corresponding sequence is identical in human VHL isoform 2 and highly conserved (94% sequence identity) in rat VHL.


Anti-VHL antibody produced in rabbit has been used in immunoblotting, immunoprecipitation and immunofluorescence.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

VHL acts as part of an E3 ubiquitin ligase that targets hypoxia-inducible factor α (HIF α) for rapid proteasomal degradation under normoxic conditions. VHL has been also identified as a key regulator of microtubule dynamic stability.

Von Hippel-Lindau tumor suppressor (VHL) acts as a tumor suppressor in some cancers. It might also play a vital role in signal transduction or cell adhesion. Mutation in the gene is associated with the development of sporadic cerebellar hemangioblastoma tumorigenesis and von Hippel-Lindau (VHL) disease. Interaction of the protein with hypoxia-inducible factor-1 (HIF-1) induces mammalian oxygen sensing.

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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