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SAB4200437 Sigma-Aldrich

Anti-EXOSC1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym: Anti-CGI-108, Anti-CSL4, Anti-Csl4p, Anti-Exosome component 1, p13, Anti-SKI4, Anti-Ski4p, Anti-hCsl4p

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Cell Biology, Cell Signaling and Neuroscience, DNA-RNA Transcription Regulators,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 1-2 μg/mL using human HeLa cells.
  western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells over-expressing human tagged-EXOSC1 (~26 kDa).
species reactivity   mouse, human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q9Y3B2
Gene Information   human ... EXOSC1(51013)
mouse ... Exosc1(66583)

Description

General description

The EXOSC1 gene is located on human chromosome 10q24.1.

The gene EXOSC1 (exosome component 1) encodes one of the nine core subunits of the exosome complex. The protein is also referred to as Csl4 and has high affinity for RNA.

Specificity

Anti-EXOSC1 recognizes human EXOSC1.

Immunogen

peptide corresponding to a sequence at the N-terminal region of human EXOSC1, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine and dog.

Application

Anti-EXOSC1 antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The exosome complex facilitates the splicing and degradation of mRNAs and non-coding RNAs. It is also essential for epigenetic gene regulation. It endogenously suppresses erythroid maturation. Within the complex, Exosc1 (exosome component 1) associates with Exosc6 to stabilize the complex. The binding of Exosc10 to Exosc1, Exosc6, and Exosc8 enhances the activity of the complex and positions Exosc1 for RNA binding.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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