• USA Home
  • SAB4200466 - Monoclonal Anti-Desmoglein 2 (DSG2) antibody produced in mouse

SAB4200466 Sigma-Aldrich

Monoclonal Anti-Desmoglein 2 (DSG2) antibody produced in mouse

~1.0 mg/mL, clone AH12.2, purified immunoglobulin

Synonym: Monoclonal Anti-ARVC10, Monoclonal Anti-ARVD10, Monoclonal Anti-CDHF5, Monoclonal Anti-CMD1BB, Monoclonal Anti-HDGC, Monoclonal Anti-desmoglein 2

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   AH12.2, monoclonal
biological source   mouse
application(s)   indirect immunofluorescence: 2.5-5.0 μg/mL using HeLa cells
  western blot: 0.5-1.0 μg/mL using HeLa total cell extracts
species reactivity   human
mol wt   antigen ~150 kDa
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1.0 mg/mL


General description

Monoclonal Anti-Desmoglein 2 (DSG2) (mouse IgG1 isotype) is derived from the hybridoma AH12.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with lipid raft enriched preparations of human T84 intestinal epithelial cells. Desmogleins are a family of cadherin proteins. There are four distinct desmoglein genes (DSG1-DSG4), each differentially expressed depending upon the type of tissue and the state of differentiation. Desmoglein-2 (DSG2) is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis and heart.


derived from the hybridoma AH12.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with lipid raft enriched preparations of human T84 intestinal epithelial cells.


Monoclonal Anti-Desmoglein 2 (DSG2) antibody produced in mouse has been used in immunoblotting and immunofluorescence.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Desmogleins play an important role in the formation of desmosomes, cell to-cell adhesions, often found in both simple and squamous epithelium. These adhesions are important for ensuring tissue integrity and regulation of paracellular movement of solutes and restricting access of luminal pathogens to underlying tissue compartments. Desmoglein-2 (DSG2) has been shown to regulate numerous cellular processes, including proliferation and apoptosis. Intracellular fragments of DSG2 promote apoptosis in colonic epithelial cells. Mutations in this gene is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC).

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?