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SAB4200473 Sigma-Aldrich

Monoclonal Anti-EpCAM ICD antibody produced in mouse

~1.0 mg/mL, clone 4A7, purified immunoglobulin

Synonym: Monoclonal Anti-DIAR5, Monoclonal Anti-EGP-2, Monoclonal Anti-EGP314, Monoclonal Anti-EGP40, Monoclonal Anti-ESA, Monoclonal Anti-HNPCC8, Monoclonal Anti-KS1/4, Monoclonal Anti-KSA, Monoclonal Anti-M4S1, Monoclonal Anti-MIC18, Monoclonal Anti-MK-1, Monoclonal Anti-TACSTD1, Monoclonal Anti-TROP1, Monoclonal Anti-epithelial cell adhesion molecule

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   4A7, monoclonal
biological source   mouse
application(s)   indirect immunofluorescence: 5-10 μg/mL using human MCF7 cells.
  western blot: 0.25-0.5 μg/mL using whole extracts of human SW480 cells.
species reactivity   mouse, human
mol wt   antigen ~40 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P16422
Gene Information   human ... EPCAM(4072)
mouse ... Epcam(17075)

Description

General description

Monoclonal Anti- EpCAM ICD (mouse IgG1 isotype) is derived from the hybridoma 4A7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. Epithelial cell adhesion molecule (EpCAM) is an epithelial transmembrane glycoprotein, composed of an ectodomain, a transmembrane domain and a cytoplasmic domain. EpCAM is frequently expressed in human malignancies, normal stem and progenitor cells, and at lower levels, in normal epithelia.

Immunogen

synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-EpCAM ICD antibody has been used in
• immunoblotting
• immunoprecipitation
• immunofluorescence

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Epithelial cell adhesion molecule′s (EpCAM) intracellular domain (ICD) fragment translocates to the nucleus after cleavage from EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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