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SAB4200494 Sigma-Aldrich

Anti-IAPP (N-terminal) antibody produced in rabbit

Antibody Enhanced Validation

~1.0 mg/mL, affinity isolated antibody

Synonym: Anti-Diabetes-associated peptide, Anti-Amylin, Anti-DAP, Anti-IAP, Anti-Islet amyloid polypeptide

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, I-IG, Primary Antibodies
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 5-10 μg/mL using formalin-fixed paraffin-embedded human pancreas.
species reactivity   human
enhanced validation   independent ( Antibodies)
Learn more about Antibody Enhanced Validation
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P10997
Gene Information   human ... IAPP(3375)

Description

General description

Islet amyloid polypeptide (IAPP), also known as amylin, is a 37 amino acid peptide hormone co-secreted with insulin from the pancreatic Β-cells. IAPP is processed from an 89-amino acid precursor pro-islet amyloid polypeptide (proIAPP) in pancreatic Β-cells that undergoes complex posttranslational modifications including protease cleavage, C-terminal amidation and formation of an intramolecular disulfide bridge to produce the mature IAPP.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminus of human pro-IAPP (GeneID 3375), conjugated to KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

Islet amyloid polypeptide (IAPP)/Amylin plays a role in glycemic regulation by slowing gastric emptying and promoting satiety, thereby preventing post-prandial spikes in blood glucose levels. The human IAPP (20-29) region that has been found to be essential to amyloid formation is thought to be a key factor in the initiation of amyloid aggregation. Amyloid deposits deriving from IAPP are commonly found in pancreatic islets of patients suffering of T2DM, or containing an insulinoma cancer. IAPP, like β-amyloid peptide associated with Alzheimer′s disease, can induce apoptosis in insulin producing β-cells, an effect that is relevant to the development of type 2 diabetes.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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