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SAB4200502 Sigma-Aldrich

Monoclonal Anti-NONO antibody produced in mouse

~1.0 mg/mL, clone NC5, purified immunoglobulin

Synonym: Monoclonal Anti-52 kDa subunit, Monoclonal Anti-54 kDa nuclear RNA- and DNA-binding protein, Monoclonal Anti-55 kDa nuclear protein, Monoclonal Anti-DNA-binding p52/p100 complex, Monoclonal Anti-NMT55, Monoclonal Anti-NRB54, Monoclonal Anti-P54, Monoclonal Anti-P54NRB, Monoclonal Anti-non-POU domain-containing octamer (ATGCAAAT) binding protein, Monoclonal Anti-p54(nrb)

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation and Expression, Antibodies to Transcription Regulators,
conjugate   unconjugated
clone   NC5, monoclonal
biological source   mouse
application(s)   indirect immunofluorescence: 1-2 μg/mL using whole extracts of mouse Hepa1-6 cells.
  western blot: 0.5-1.0 μg/mL using whole extracts of mouse Hepa1-6 cells.
species reactivity   rat, mouse
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q99K48
Gene Information   mouse ... Nono(53610)
rat ... Nono(317259)

Description

Immunogen

Synthetic peptide corresponding to a sequence at the C-terminal region of mouse NONO, conjugated to KLH.1 The corresponding sequence is identical in rat and differs by a single amino acids in human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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