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SAB4200518 Sigma-Aldrich

Anti-SLC1A1 antibody produced in rabbit

Antibody Enhanced Validation

~1.0 mg/mL, affinity isolated antibody

Synonym: Anti-EAAT3, Anti-solute carrier family 1, member 1, EAAC1

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Neurobiology, Antibodies to Neural Proteins, Neuroscience,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin-embedded human small intestine.
  western blot: 1.5-3.0 μg/mL using Neuro-2a cell extracts.
species reactivity   mouse, human
mol wt   antigen ~70 kDa
enhanced validation   recombinant expression
Learn more about Antibody Enhanced Validation
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P43005
Gene Information   human ... SLC1A1(6505)
mouse ... Slc1a1(20510)
rat ... Slc1a1(25550)

Description

General description

SLC1A1 (also known as EAAC1, EAAT3) is localized at the postsynaptic site of neurons. It is expressed at moderate levels in the adult brain, mainly in the hippocampus, basal ganglia and olfactory bulb and is considered to make a minor contribution to glutamate removal from the synapse.

Immunogen

synthetic peptide corresponding to a sequence near the C-terminus of human SLC1A1, conjugated to KLH. The corresponding sequence is identical in mouse and rat SLC1A1.

Application

Anti-SLC1A1 antibody produced in rabbit has been used in immunohistochemistry and immunoblotting.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

SLC1A1 regulates the developmental effects of glutamate. It is highly regulated by neural activity and intracellular signaling pathways involving mainly PKCa, PI3K, and glial factors, resulting in rapid changes in the trafficking of the transporter and in its membrane location.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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