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SAB4200541 Sigma-Aldrich

Anti-ADAR1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym: Anti-136 kDa double-stranded RNA-binding protein, Anti-interferon-induced protein 4, Anti-DRADA, Anti-DSH, Anti-G1P1, Anti-IFI-4, Anti-IFI4, Anti-K88DSRBP, Anti-P136, Anti-adenosine deaminase acting on RNA 1-A, Anti-adenosine deaminase, RNA-specific, Anti-double-stranded RNA-specific adenosine deaminase

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Properties

Related Categories AD-AD, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation and Expression,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 10-20 μg using lysates of human HepG2 or HEK-293T cells.
  indirect immunofluorescence: 5-10 μg/mL using human HepG2 cells.
  western blot: 2-4 μg/mL using whole extracts of human HEK-293T cells.
species reactivity   human
mol wt   antigen mol wt ~110 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P55265
Gene Information   bovine ... ADAR(505134)
human ... ADAR(103)
mouse ... Adar(56417)
rat ... Adar(81635)

Description

Immunogen

synthetic peptide corresponding to an internal s of human ADAR1, conjugated to KLH. The corresponding sequence is identical in mouse, rat, bovine, pig and monkey.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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