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  • SAB4200547 - Monoclonal Anti-IDH1 (R132S) antibody produced in mouse

SAB4200547 Sigma-Aldrich

Monoclonal Anti-IDH1 (R132S) antibody produced in mouse

~1.0 mg/mL, clone SMab-1, purified immunoglobulin

Synonym: Monoclonal Anti-NADP(+)-specific ICDH, Monoclonal Anti-IDCD, Monoclonal Anti-IDH, Monoclonal Anti-IDP, Monoclonal Anti-IDPC, Monoclonal Anti-NADP-dependent isocitrate dehydrogenase, cytosolic, Monoclonal Anti-Oxalosuccinate decarboxylase, Monoclonal Anti-PICD, Monoclonal Anti-isocitrate dehydrogenase 1 (NADP+), soluble, NADP-dependent isocitrate dehydrogenase, peroxisomal

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, I-IG, Primary Antibodies
conjugate   unconjugated
clone   SMab-1, monoclonal
biological source   mouse
application(s)   immunoblotting: 0.25-0.5 μg/mL using using total cell extracts of HEK-293T cells overexpressing IDH1R132S
species reactivity   human
mol wt   antigen ~43 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   O75874
Gene Information   human ... IDH1(3417)


General description

Monoclonal Anti-IDH1 (R132S) (mouse IgG1 isotype) is derived from the hybridoma SMab1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized by a peptide. Isocitrate dehydrogenase 1 (IDH1) is a homodimeric, nicotinamide adenine dinucleotide phosphate (NADP)-specific enzyme. It is localized in the peroxisomes and cytoplasm. IDH1 is mapped on the human chromosome at 2q34.


Monoclonal Anti- IDH1 (R132S) recognizes only human R132S mutated IDH1 and does not cross react with others.


immunized a peptide corresponding to the mutation R132S of human IDH1. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).


Monoclonal Anti-IDH1 (R132S) antibody produced in mouse may be used in:
• immunoblotting
• immunofluorescence
• immunohistochemistry

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Isocitrate dehydrogenase (IDH) plays an important role in catalyzing the oxidative decarboxylation of isocitrate to α-ketoglutarate (αKG). Mutations in this gene are associated with several cancers, such as glioblastoma, acute myeloid leukemia (AML) and chondrosarcoma. Mutation in IDH1 specific to Arg132 results in the formation of 2-hydroxyglutarate (2HG) instead of αKG. 2HG hinders DNA and histone methylation resulting in alteration of gene transcription.

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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