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  • SAB4200547 - Monoclonal Anti-IDH1 (R132S) antibody produced in mouse

SAB4200547 Sigma

Monoclonal Anti-IDH1 (R132S) antibody produced in mouse

~1.0 mg/mL, clone SMab-1, purified immunoglobulin

Synonym: Monoclonal Anti-NADP(+)-specific ICDH, Monoclonal Anti-IDCD, Monoclonal Anti-IDH, Monoclonal Anti-IDP, Monoclonal Anti-IDPC, Monoclonal Anti-NADP-dependent isocitrate dehydrogenase, cytosolic, Monoclonal Anti-Oxalosuccinate decarboxylase, Monoclonal Anti-PICD, Monoclonal Anti-isocitrate dehydrogenase 1 (NADP+), soluble, NADP-dependent isocitrate dehydrogenase, peroxisomal



Related Categories Alphabetical Index, Antibodies, I-IG, Primary Antibodies
species reactivity   human
application(s)   immunoblotting: 0.25-0.5 μg/mL using using total cell extracts of HEK-293T cells overexpressing IDH1R132S
clone   SMab-1, monoclonal
concentration   ~1.0 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~43 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... IDH1(3417)
biological source   mouse
conjugate   unconjugated



immunized a peptide corresponding to the mutation R132S of human IDH1. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Price and Availability

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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