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  • SAB4200571 - Anti-Neuron-Specific Enolase (NSE), Mouse monoclonal

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SAB4200571 Sigma-Aldrich

Anti-Neuron-Specific Enolase (NSE), Mouse monoclonal

clone NSE-P1, purified from hybridoma cell culture

Synonym: Anti-2-phospho-D-glycerate hydrolyase, Anti-Enolase 2 (gamma, neuronal), Anti-NSE, Anti-Neural enolase, Anti-gamma-enolase, Monoclonal Anti-2-phospho-D-glycerate hydro-lyase

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Neurobiology, Antibodies to Neural Enzymes, NE-NI,
conjugate   unconjugated
clone   NSE-P1, monoclonal
biological source   mouse
application(s)   immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human cerebellum.
  western blot: 0.5-1.0 μg/mL using NTERA-2 (NT2/D1) total cell extracts.
species reactivity   human, rat, mouse
mol wt   antigen ~47 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P09104
Gene Information   human ... ENO2(2026)

Description

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human NSE.1 The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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