• USA Home
  • SAB4200585 - Monoclonal Anti-ERGIC-53 antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
SAB4200585 Sigma-Aldrich

Monoclonal Anti-ERGIC-53 antibody produced in mouse

~1.0 mg/mL, clone ERGIC-3, purified immunoglobulin

Synonym: Monoclonal Anti-ER-Golgi intermediate compartment 53 kDa protein, Monoclonal Anti-ERGIC53, Monoclonal Anti-F5F8D, Monoclonal Anti-Gp58, Monoclonal Anti-Intracellular mannose-specific lectin MR60, Monoclonal Anti-LMAN1, Monoclonal Anti-Lectin mannose-binding 1

  •  NACRES NA.41

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   ERGIC-3, monoclonal
biological source   mouse
application(s)   immunoblotting: 1-2 μg/mL using whole extracts of human HeLa cells.
species reactivity   mouse, rat, human
mol wt   antigen ~58 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P49257
Gene Information   human ... LMAN1(3998)
mouse ... Lman1(70361)
rat ... Lman1(116666)

Description

General description

Endoplasmic reticulum-Golgi intermediate compartment 53kDa protein (ERGIC-53), a nonglycosylated, hexameric type I integral membrane protein is also known as LMAN1 (lectin, mannose-binding 1). This gene is located on human chromosome 18q21.32.

Immunogen

synthetic peptide corresponding to an internal sequence of human ERGIC-53, conjugated to KLH. The corresponding sequence is identical in mouse, rat, bovine and monkey.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Endoplasmic reticulum-Golgi intermediate compartment protein 53kDa protein (ERGIC-53) plays an important role in the proliferation of arenavirus, coronavirus and filovirus. This intracellular cargo receptor enables the anterograde passage of very few glycoprotein ligands in the early exocytic pathway. Mutations in ERGIC-53 (LMAN1) gene results in the deficiency of factor V and factor VIII.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?