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  • SAB4200635 - Monoclonal Anti-C19orf10 antibody produced in mouse

SAB4200635 Sigma-Aldrich

Monoclonal Anti-C19orf10 antibody produced in mouse

clone 15.3.3, purified from hybridoma cell culture

Synonym: IL25, IL27, IL27w, Stromal cell-derived growth factor SF20, UPF0556 protein C19orf10, myeloid-derived growth factor (MYDGF)

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, C1-C19, Primary Antibodies
clone   15.3.3, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunoblotting: suitable
  immunofluorescence: suitable
species reactivity   human, canine, bovine
mol wt   ~17 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   Q969H8
Gene Information   human ... MYDGF(56005)


General description

Monoclonal Anti-C19orf10 (mouse IgG2a isotype) is derived from the hybridoma 15.3.3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the internal region of human C19orf10, conjugated to KLH. C19orf10 (chromosome 19 open reading frame 10) also known as IL25 (Interleukin - 25) and Stromal cell-derived growth factor SF20, is a secreted protein. This protein is an FLS (fibroblast-like synoviocytes)-derived protein and is discharged into the synovial fluid.


synthetic peptide corresponding to a sequence at the internal region of human C19orf10.


Monoclonal Anti-C19orf10 antibody has been used in
• immunoblotting
• immunocytochemistry
• immunofluorescence
• flow cytometry

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

C19orf10 (chromosome 19 open reading frame 10) was overexpressed in approximately two-thirds of hepatocellular carcinoma (HCC) tissues compared to the adjacent non-cancerous liver tissues. It also regulates HCC cell line proliferation by activating the Akt/mitogen-activated protein kinase pathways.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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