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SAB4200660 Sigma-Aldrich

Monoclonal Anti-GOLPH3 antibody produced in mouse

clone GOL3-1, purified from hybridoma cell culture

Synonym: GOPP1, GPP34, MIDAS, golgi phosphoprotein 3 (coat-protein)

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Properties

Related Categories Alphabetical Index, Antibodies, GM-GO, Primary Antibodies
clone   GOL3-1
biological source   mouse
application(s)   immunoblotting: 2.5-5 μg/mL using using whole extracts of rat PC-12 cells.
  immunofluorescence: 2.5-5 μg/mL using mouse 3T3 cells
  immunoprecipitation (IP): 5-10 μg using using lysates of human HeLa cells
species reactivity   human, mouse, rat
mol wt   antigen ~34 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   Q9H4A6
Gene Information   human ... GOLPH3(64083)
mouse ... Golph3(66629)
rat ... Golph3(78961)

Description

General description

GOLPH3 (golgi phosphoprotein 3) is an abundant phosphatidylinositol-4-phosphate (PtdIns4P)-interacting protein which is conserved from yeast to humans. This protein is localized to Golgi through PtdIns4P. This gene is localized to human chromosome 5p13. GOLPH3 has a molecular weight of 34kDa.

Monoclonal Anti-GOLPH3 (mouse IgG1 isotype) is derived from the hybridoma GOL3-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. GOLPH3 (Golgi phosphoprotein 3) is a peripheral membrane protein of the Golgi stack that localizes to the trans-Golgi network. GOLPH3 is conserved from yeast to human. This gene was described as an oncogene that is commonly amplified in human cancer and in cancer cell lines.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminal region of human Golph3

Application

Monoclonal Anti-GOLPH3 antibody produced in mouse has been used in:
• immunoblotting
• immunoprecipitation
• immunofluorescence

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

GOLPH3 (golgi phosphoprotein 3) links Golgi membrane to actin cytoskeleton by interacting with MYO18A, which is an unconventional myosin. This, in turn, is crucial for Golgi architecture and vesicle trafficking. It is an oncogene, which is susceptible to amplification in cancers, and is responsible for controlling the response to the anti-cancer drug, rapamycin. It is thought to function as a coat protein during Golgi trafficking.

GOLPH3 has a role in Golgi trafficking and morphology. It interacts with the unconventional myosin MYO18A, linking Golgi membranes to the actin cytoskeleton. GOLPH3 is also required for Golgi function. Overexpression of GOLPH3 is correlated with hyperactivation of mTOR signaling, in human cancer cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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