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SAB4200752 Sigma-Aldrich

Anti-Nuclease P1 antibody produced in rabbit

Ig fraction of antiserum, buffered aqueous solution

Synonym: Anti-Deoxyribonuclease P1, Anti-Endonuclease P1

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, NS-NZ, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunoblotting: 1:10,000-1:20,000 using Nuclease P1 from Penicillium citrinum
mol wt   ~30 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   Ig fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P24289

Description

General description

Nuclease P1 (Nuclease 5′-Nucleotidehydrolase, 3′- Phosphohydrolase, EC 3.1.30.1), also known as deoxyribonuclease P1 or endonuclease P1, is a zinc dependent endonuclease. It is isolated from Penicillium citrinum.

Immunogen

Nuclease P1 from Penicillium citrinum

Application

Anti-Nuclease P1 antibody produced in rabbit has been used in immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

Nuclease P1 catalyzes the hydrolysis of 3′-5′ phosphodiester bonds in RNA and single-stranded DNA and 3′-phosphomonoester bonds in mono- and oligonucleotides terminated by a 3′-phosphate group without apparent bases specificity. The enzyme is used as a component of nuclease cocktail to generate single-stranded oligodeoxynucleotide (ODNs) samples for mass spectroscopy research for characterization of oligodeoxyribonucleotides. Nuclease P1 has also been widely used in the food industry to enhance or create flavor.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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