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  • SAB4200753 - Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal

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SAB4200753 Sigma-Aldrich

Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal

clone AK-11, purified from hybridoma cell culture

Synonym: Anti-Protein Kinase B, Anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1

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Properties

Related Categories Alphabetical Index, Antibodies, PH-PI, Primary Antibodies
clone   AK-11, monoclonal
biological source   mouse
application(s)   immunoblotting: 1-2 μg/mL using human breast cancer cell line MCF-7 extract
  immunofluorescence: 1-2 μg/mL using mouse embryo fibroblast NIH-3T3 cells
  immunoprecipitation (IP): 2-4 μg/test using whole extract of mouse embryo fibroblast NIH-3T3 cell line
species reactivity   mouse, rat, chicken, human
mol wt   mol wt ~56 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
isotype   IgG2a
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P31749
Gene Information   human ... AKT1(207)

Description

Immunogen

Synthetic peptide corresponding to human AKT1 pThr450

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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