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SAB4200757 Sigma-Aldrich

Anti-PLA2R antibody, Mouse monoclonal

clone 12-6-5, purified from hybridoma cell culture

Synonym: Anti-180 kDa secretory phospholipase A2 receptor, Anti-C-type lectin domain family 13 member C, Anti-M-type receptor, Anti-PLA2-R, Anti-PLA2R, Anti-Secretory phospholipase A2 receptor

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Properties

clone   12-6-5, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunoblotting: 1-2 μg/mL using partial recombinant human PLA2R1 protein (predicted ~150kDa)
  immunofluorescence: suitable
  immunohistochemistry: suitable
species reactivity   human
mol wt   ~180 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
isotype   IgG2b
antibody product type   primary antibodies
Quality Level   200
concentration   ~1 mg/mL
UniProt accession no.   Q13018
Gene Information   human ... PLA2R1(22925)

Description

General description

Anti-PLA2R antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the 12-6-5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with recombinant fragment of human PLA2R corresponding to extracellular sequence NC3. Secretory phospholipase A2 receptor (PLA2R) is 180 kDa and is a type I transmembrane glycoprotein member of the mannose receptor (MR) family. Large extracellular glycosylated region comprising an N-terminal cysteine-rich domain (CysR), a fibronectin-like type II domain (FnII), and eight to ten C-type lectin-like domains (CTLD).

Immunogen

Human PLA2R extracellular sequence N-C3 (containing domains N-terminal Cysteine rich-FibII-CTLD1-CTLD2-CTLD3)

Application

Anti-PLA2R antibody has been used in
• immunoblotting
• immunofluorescence
• flow Cytometry
• immunohistochemistry

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Biochem/physiol Actions

Secretory phospholipase A2 receptor (PLA2R) acts as a receptor for secretory phosholipase A2 (sPLA2) allowing its removal from circulation, thus regulating its biological effect. PLA2R is also involved in clearance followed by suppression of their potent enzymatic activities of secretory phospholipase A2 (sPLA2) from the blood, including group sPLA2-X and a snake venom sPLA2. The soluble circulating form of PLA2R is constitutively present as an endogenous inhibitor of sPLA2s. PLA2R is expressed in kidney podocytes of normal human glomeruli, in idiopathic membranous nephropathy (IMN) patients PLA2R is colocalized to immune deposits in glomeruli along with IgG4.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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