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  • SAB4200768 - Anti-Human IgG1-Peroxidase antibody, Mouse monoclonal

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SAB4200768 Sigma-Aldrich

Anti-Human IgG1-Peroxidase antibody, Mouse monoclonal

clone 8c/6-39, purified from hybridoma cell culture

Synonym: Anti-Human immunoglobulin G1

  •  NACRES NA.46

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Properties

biological source   mouse
antibody product type   primary antibodies
clone   8c/6-39, monoclonal
form   lyophilized
species reactivity   human
concentration   ~2 mg/mL
application(s)   ELISA: 1:8,000-1:16,000 using 5 μg/ml human IgG1 for coating
isotype   IgG2a
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

General description

Human IgGs consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5, and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties.Monoclonal Anti-Human IgG1 specifically recognizes Human IgG1. The antibody shows no cross-reactivity with human IgG2, IgG3, or IgG4. This clone has been established as a useful human IgG1 specificity standard by the WHO/IUIS.

Monoclonal Anti-Human IgG1 (mouse IgG2a isotype) is derived from the 8c/6-39 hybridoma (also known as HP6019), produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with the Fc fragment of a human IgG1 myeloma protein.

Immunogen

The Fc fragment of a human IgG1 myeloma protein

Application

The antibody is recommended to use in various immunological techniques, including ELISA.

Biochem/physiol Actions

The immunoglobin G (IgG) subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies. Human IgG1 is the main antibody produced against tetanus toxoid antibodies. Elevation of IgG1 levels has been found in the cerebral spinal fluid of patients with multiple sclerosis.

Physical form

Supplied as a lyophilized powder

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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