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  • SAB4200769 - Anti-Human IgG3-Peroxidase antibody, Mouse monoclonal

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SAB4200769 Sigma-Aldrich

Anti-Human IgG3-Peroxidase antibody, Mouse monoclonal

clone HP-6050, purified from hybridoma cell culture

Synonym: Anti-Human immunoglobulin G3

  •  NACRES NA.46

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Properties

Quality Level   200
biological source   mouse
antibody product type   primary antibodies
clone   HP-6050, monoclonal
form   lyophilized powder
species reactivity   human
concentration   ~2 mg/mL
application(s)   direct ELISA: 1:80,000-1:160,000 using 2 μg/mL human IgG3 for coating.
isotype   IgG1
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

General description

Human IgG consist of four subclasses (1-4) that can be recognized by the antigenic difference in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens responses give rise to IgG1 and IgG3 antibodies. IgG1 and IgG3 are the only subclasses capable of adherence to mononuclear phagocytes and are recognized readily by the Fc receptors on various reticulo-endothelial cells, while IgG2 and IgG4 are far less efficient. The abundance of the different IgG subclasses in the bloodstream varies with age, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels rise more slowly, reaching adult levels at about 10 years of age. Serum IgG subclass deficiencies have been recorded for different patient groups. IgG3 deficiency has been associated with a patient’s history of recurrent infectious that may lead to chronic lung disease. Decreased IgG3 levels are frequently associated with IgG1 deficiency. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the related immunological processes and may assist in the diagnosis of various disorders.

Immunogen

Human IgG3 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Application

Direct ELISA: a working dilution of 1:80,000-1:160,000 is recommended using 2 μg/mL human IgG3 for coating.

Physical form

Supplied as a lyophilized powder.

Other Notes

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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