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SAB4200774 Sigma-Aldrich

Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal

clone 13C4, purified from hybridoma cell culture

Synonym: Anti-SLT-1 B subunit, Anti-SLT-1b, Anti-Shiga-like toxin 1 subunit B, Anti-Verocytotoxin 1 subunit B, Anti-Verotoxin 1 subunit B, Anti-stxB

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Properties

Related Categories Antibodies, Microbiome Antibodies More...
Quality Level   200
biological source   mouse
antibody product type   primary antibodies
clone   13C4, monoclonal
description   Research area: Microbiome
form   buffered aqueous solution
mol wt   ~7 kDa
species reactivity   E. coli
concentration   ~1.0 mg/mL
application(s)   flow cytometry: 2.5-5 μg/test using human RAMOS cells pretreated with recombinant Shiga toxin 1, B subunit
  immunoblotting: 1-2 μg/mL using purified recombinant Shiga Toxin 1-B subunit produced in E. coli (using PVDF membrane)
  immunoprecipitation (IP): suitable
isotype   IgG1
UniProt accession no.   Q7BQ98
shipped in   dry ice
storage temp.   −20°C

Description

General description

Monoclonal Anti-Shiga Toxin 1, B Subunit (mouse IgG1 isotype) is derived from the 13C4 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a purified Shiga-like toxin from E. coli H30. Shiga toxins consist of two polypeptides, an A chain and a B chain non-covalently associate with an apparent stoichiometry of one A and five B chains to form the holotoxin.

The Shiga toxins are a family of related protein toxins secreted by certain types of bacteria. Shiga toxin (Stx) is produced by Shigella dysenteriae; whereas, the Shiga-like toxins, Stx1 and Stx2, with a few known isoforms, are secreted by specific strains of Escherichia coli named Shiga-toxin-producing E. coli (STEC) such as E. coli O157:H7. The latter may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications.

Specificity

Monoclonal Anti-Shiga Toxin 1, B Subunit antibody specifically recognizes the B subunit of Shiga holotoxin.

Immunogen

purified Shiga-like toxin from E.coli H30

Application

Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal has been used in:
• immunoblotting
•  flow cytometry
•  immunoprecipitation

Biochem/physiol Actions

Shiga-toxin (Stx) produced by E. coli O157:H7 may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications. All the Stx isoforms have similar structure and mechanism of action. The catalytic A subunit has RNA N-glycosidase activity that inhibits eukaryotic protein synthesis. The B subunits form a pentamer, which recognizes and binds to the functional cell-surface receptor globotriaosylceramide [Gb3, Gala(1-4)-Galb (1-4)-Glcb1-ceramide]. Gb3 is overexpressed in membranes of numerous tumor cells, Therefore, STxB binding to Gb3 receptors may be useful for cell-specific vectorization, labeling, and imaging purposes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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