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SAB4200813 Sigma-Aldrich

Anti-α Actinin antibody, Mouse monoclonal

clone BM-75.2, purified from hybridoma cell culture

Synonym: Alpha-actinin cytoskeletal isoform, Alpha-actinin-1, F-actin cross-linking protein, Non-muscle alpha-actinin-1

  •  NACRES NA.41

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Properties

clone   BM-75.2, monoclonal
biological source   mouse
application(s)   immunoblotting: 0.5-1 μg/mL using human HeLa cells extract.
  immunofluorescence: 5-10 μg/mL using human foreskin fibroblast Hs68 cells.
species reactivity   human
mol wt   ~100 kDa
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   purified from hybridoma cell culture
isotype   IgM
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P12814
Gene Information   human ... ACTN1(87)

Description

General description

Monoclonal Anti- α Actinin (mouse IgM isotype) is derived from the hybridoma BM-72.5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells. α-Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle, α-actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle, α-actinin has been detected in dense bodies and plaques characteristic of the tissue.

Specificity

Monoclonal Anti-α-Actinin specifically recognizes α-Actinin from human1, mouse2, rat3, chicken4, monkey, canine and bovine origin. 

Immunogen

Cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells.

Application

Anti-α Actinin antibody may be used in various immunochemical techniques including Immunoblot (~100 kDa), Immunohistochemistry and Immunofluorescence. Monoclonal Anti-α-Actinin may be used for immunofluorescent localization of α-actinin in cultured cells and tissues, for immunofluorescent labeling of skeletal muscle (normal and pathological) in order to detect its organizational state, studies of membrane anchorage sites and immunochemical identification of α-actinin by immunoblotting analysis.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

α-actinin has extensive proteins association with actin containing stress fibers, in particular with their membrane bound termini.

α-actinin modulates muscle contraction and has a role in cytoskeletal organisation. α-actinin acts as an actin crosslinker and scaffold. Mutations in this gene has been linked to heterogeneous hypertrophic cardiomyopathy and juvenile onset atrial fibrillation.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Small Pack Size Primary Antibodies

Economical means affordable options when exploring new antibodies. To provide you with convenient and economical access to our highly characterized antibodies, we now offer small pack size antibodies...
Keywords: Apoptosis, Cancer, Enzyme-linked immunosorbent assay, Epigenetics, Flow cytometry, Growth factors, Immunocytochemistry, Immunohistochemistry, Immunology, Individual protein Immunoprecipitation, Inflammation, Neuroscience, Research areas, Western blot

Peer-Reviewed Papers
15

References

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